rabbit anti cd86 antibody  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: Circadian disruption exacerbates MASH by reducing Akkermansia muciniphila via the FXR-CYP7A1-bile acid axis

doi: 10.1016/j.isci.2026.115397

Figure Lengend Snippet: Circadian disruption promotes inflammation, lipid accumulation, and fibrosis in MASH mice (A) The relative mRNA expression of lipid-metabolism-related genes ( Srebp , Fasn , and Acaca ), inflammation-related genes ( Cxcl2 , Ccl2 , and Nos2 ), and fibrosis-related genes ( Col3a1 , Col4a1 , and α-Sma ) in MASH with or without circadian disruption, n = 6. (B) Flow cytometry analysis of the abundance of macrophages (CD11b + F4/80 + ), M1 cells (CD86 + CD206 − ), and M2 cells (CD86 − CD206 + ) in the liver in each group, n = 6. (C) Comparison of M1/M2 polarization in the liver in each group by CD86/CD206 detected by immunohistochemistry. Scale bars: 100 μm (10×). (D) Serum IL-6, IL-1β, and TNF-α in each group, n = 6. (E) The relative expression level of the oxidative stress indicator ROS, the content of GSH, and the relative mRNA expression level of Hif-1α related to them in each group, n = 6. (F) The total number of genes detected by liver transcriptomics, as well as the number of genes that are relatively upregulated and downregulated in the two groups. (G) NMDS plot of the main components of liver transcriptomics in each group, n = 4. (H) Reactome analysis results (barplot) of liver transcriptomics in the two groups. (I) Reactome analysis results (dotplot) of liver transcriptomics in the two groups. Data are expressed as mean ± SD ( n = 4–6), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, compared with the MASH group. p values were calculated using unpaired t test. Srebp , sterol regulatory element-binding protein; Fasn , fatty acid synthase; Acaca , acetyl-CoA carboxylase; Cxcl2 , chemokine (C–X–C motif) ligand 2; Ccl2 , chemokine (C–C motif) ligand 2; Nos2 , nitric oxide synthase 2; Col3a1 , collagen alpha-1 (III) chain; Col4a1 , collagen alpha-1 (IV) chain; α-Sma , α-smooth muscle actin; M1, M1 polarization of macrophages; M2, M2 polarization of macrophages; CD11b, marker of mouse macrophages; F4/80, marker of mouse macrophages; CD86, marker of M1 polarization of mouse macrophages; CD206, marker of M2 polarization of mouse macrophages; IL-6, interleukin-6; IL-1β, interleukin-1 beta; TNF-α, tumor necrosis factor-alpha; ROS, reactive oxygen species; GSH, glutathione; Hif-1α , hypoxia inducible factor-1 alpha; NMDS, non-metric multidimensional scaling.

Article Snippet: Rabbit anti-CD86 antibody , Cell Signaling Technology , Cat# 91882; RRID: AB_2797422.

Techniques: Disruption, Expressing, Flow Cytometry, Comparison, Immunohistochemistry, Transcriptomics, Binding Assay, Marker

Journal: Kidney International Reports

Article Title: Pauci-Immune Endocapillary Proliferative Glomerulonephritis With Glomerular M2 Macrophage Infiltration

doi: 10.1016/j.ekir.2026.103791

Figure Lengend Snippet: Multiplex immunofluorescence staining results (Case 3). (a) Macrophage subtypes and their distribution patterns. (b) CD86+ macrophages are sparsely distributed within the renal interstitium. (c–e) CD163+ and CD206+ macrophages are abundantly present in the interstitium, whereas CD163+ macrophages show less infiltration within the glomeruli. The scale bar in the figure represents 20 μm. DAPI, 4',6-diamidino-2-phenylindole.

Article Snippet: Formalin-fixed paraffin-embedded renal tissue sections (3.5 μm) were subjected to multiplex IF staining using the following primary antibodies: CD68 (ab955, pan-macrophage marker, Abcam), CD163 (ab182422, M2 macrophage marker, Abcam), CD86 (91882S, M1 macrophage marker, Cell Signaling Technology, Danvers, MA), CD206 (24595S, M2 macrophage marker, Cell Signaling Technology), CD56 (99746S, Cell Signaling Technology), CD3 (ab11089, Abcam), and CD8 (ab199016, Abcam).

Techniques: Multiplex Assay, Immunofluorescence, Staining